A newsgroup question caught my attention recently and I thought I'd employ the above sheep to help solve the problem. I met them on a walk with my wife on holiday in Scotland and, while they know next to nothing about Visio, they make a useful sample image for this post.
The question - "How do you highlight part of an image in Visio"?
Whilst you can get to a number of image properties via Format / Picture... and the ShapeSheet, spot highlighting (or any other localized pixel-level manipulation) isn't one of them...